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Literature summary extracted from

  • Wang, H.; Wu, J.; Chia, J.; Yang, C.; Wu, Y.; Juang, R.
    Phytochelatin synthase is regulated by protein phosphorylation at a threonine residue near its catalytic site (2009), J. Agric. Food Chem., 57, 7348-7355.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.2.15 Cd2+ the activity of PCS requires Cd and is enhanced by protein phosphorylation Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.15 the coding sequences of full-length PCS1, PCS-N, residues 1-221, and PCS-C, residues 222-485, are cloned into the vector pGEM-T-Easy and subsequently into pET28b for expression in Escherichia coli BL21DE3 cells Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.15 R183a AtPCS1 mutant, Arg183 is critical to the activity of PCS Arabidopsis thaliana
2.3.2.15 R183a AtPCS1-N mutant, Arg183 is critical to the activity of PCS Arabidopsis thaliana
2.3.2.15 T49A AtPCS1 mutant, Thr49 is the only residue to be phosphorylated Arabidopsis thaliana
2.3.2.15 T49A AtPCS1-N mutant, Thr49 is the only residue to be phosphorylated Arabidopsis thaliana
2.3.2.15 Y55A AtPCS1 mutant Arabidopsis thaliana
2.3.2.15 Y55A AtPCS1-N mutant Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.15 26700
-
 AtPCS1-N, residues 1-221, theoretical, verified by SDS-PAGE and Western Blot analysis Arabidopsis thaliana
2.3.2.15 31400
-
 AtPCS1-C, residues 222-485, theoretical, verified by SDS-PAGE and Western Blot analysis Arabidopsis thaliana
2.3.2.15 56300
-
 AtPCS1, residues 1-485, theoretical, verified by SDS-PAGE and Western Blot analysis Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.15 glutathione + [Glu(-Cys)]n-Gly Arabidopsis thaliana
-
 Gly + [Glu(-Cys)]n+1-Gly
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.15 Arabidopsis thaliana Q9S7Z3
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.2.15 phosphoprotein PCS1 expressed in Escherichia coli is phosphorylated at a Thr residue near its catalytic site Arabidopsis thaliana

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.15 using a HisTrap FF column Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.15 seedling
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.15 glutathione + [Glu(-Cys)]n-Gly
-
 Arabidopsis thaliana Gly + [Glu(-Cys)]n+1-Gly
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.15 AtPCS1
-
 Arabidopsis thaliana
2.3.2.15 gamma-glutamyl-cysteine transpeptidase
-
 Arabidopsis thaliana
2.3.2.15 PCS
-
 Arabidopsis thaliana
2.3.2.15 PCS1
-
 Arabidopsis thaliana
2.3.2.15 phytochelatin synthase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.15 37
-
 activity assay Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.15 7.6
-
 activity assay Arabidopsis thaliana